Development of an indigenous chip for testing antibiotic sensitivity of pathogens found in the human urinary tract.

at Genomics Laboratory, BITS Pilani, Hydrabad Campus (November, 2013 - December, 2013)

 

Fellow Collaborators:
Dr. Suman Kapur, Dean International Programmes and Collaborations, Senior Professor BITS Pilani, Hyderabad Campus
Dr. Anuradha Pal, Post-doctoral Fellow, Biological Sciences Dept., BITS Pilani Hyderabad Campus
Ms. Shivani Gupta, PhD Student, Biological Sciences Dept., BITS Pilani Hyderabada Campus

Ms. D.V. Padmavati, PhD Student, Biological Sciences Dept., BITS Pilani Hyderabad Campus
Mr. Raghavender Sahdev, Undergraduate Student, BITS Pilani, Hyderabad Campus

 

 

This involved coding for the machine, circuit design and mechanical framing of the assembly of an antibiotic finder for cultured bacteria to detect urine samples. Three different mechanisms were designed. The coding was done on arduino and required the use of various interfaces with sensors, stepper motors, handling interrupts etc. My job was also to come up with an algorithm based on prior data given to generate a formula for Tubidity of the urine essay given. It involved finding similarity between given data 

 

A point-of-care device for testing antibiotic sensitivity of pathogens found in human urinary tract: The developed device offers

a) Ease of operation and analysis of results

b) rapid results at the bedside or in doctors chambers/lab in less than two hours

c) reliability comparable to conventional disc assay for antibiotic sensitivity of pathogens

d) Affordable low cost per test.  

 

UTI alone leads to more than 8 million office visits and over a million hospitalizations each year in USA alone.  The device provides for growth of pathogens in a specially designed medium ensuring rapid growth with release of a product linearly related to the number of bacteria present.  In the instance of the bacteria being sensitive to any given antibiotic the chromogen is not released.  The released chromogen is read out using a specially fabricated readout-machine, based on a color to frequency conversion, giving both an alphanumeric display on a screen and a print out for permanent record of the report.  The preloaded antibiotic panel comes in two sets and allows for screening fourteen antibiotics with provision for seven more.

 

The machine functionality:

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

* funded by NP MASS DRDO

** PATENT Granted

** I was given 10,000  INR as stipend for my contribution to the project
** Idea presented at Shrishti competition sponsored by CFI for Effective Health care solution. My team came 1st in the competition and won cash prize of 30,000 INR
 

*** The start-up won the frist prize worth Rs. 500,000 INR at the BEST India 2014 competition organised by Department of Biotechnology, Govt. of India. I got 20% of this amount as the creator of this market ready device.